Molecular detention of efflux pumps in Pseudomonas aeruginosa obtained from different clinical cases

150 samples were collected from different clinical sources Al-Diwaniyah city hospitals September 2021 to January 2022. The grown on Maconkey agar and blood medium incubated at 37°C for 24 hours. Bacterial isolates diagnosed through culture characteristics, microscopic examination biochemical tests, as 25 belonging p. aeruginosa. phenotypic detection of these was conducted it found that 16 contain efflux pumps, after which the genetic done by PCR technique. subjected mexAB-OprM pump using technique, replication product migrated in agarose gel, observed 93.75 %) possess mexA gene encoding above pump, (43.75%) oprM also.